Development of a strain-specific PCR as a diagnostic tool for surveillance, detection, and monitoring of vancomycin-resistant Enterococcus faecium during outbreak

Table 4 Sensitivity of the Marker4 TaqMan assay in detecting E. faecium ST117-CT469 DNA in the presence of human DNA. This table presents the cycle quantification (Ct) values obtained from the Marker4 TaqMan assay for various concentrations of E. faecium DNA with 50 Ng of human DNA included where indicated. Each concentration was tested in triplicate and the mean value was used to assess the assay sensitivity

Human DNA amount in PCR reaction	E. faecium ST117-CT469 DNA amount in PCR reaction	Replicate 1 Ct value	Replicate 2 Ct value	Replicate 3 Ct value	Mean
0 ng	50 ng	12.61	12.25	12.46	12.44
50 ng	50 ng	13.07	12.7	13.03	12.93
50 ng	5 ng	16.29	16.45	16.14	16.29
50 ng	500 pg	19.86	20.12	19.86	19.94
50 ng	50 pg	23.45	23.33	23.27	23.35
50 ng	5 pg	27.1	27.01	27.02	27.04
50 ng	500 fg	30.25	30.65	30.37	30.42
50 ng	50 fg	35.41	35.3	35.2	35.31
50 ng	5 fg	39.02	40.12	Fluorescence signal not detected	39.57

ISSN: 2047-2994