Development of a strain-specific PCR as a diagnostic tool for surveillance, detection, and monitoring of vancomycin-resistant Enterococcus faecium during outbreak

Sabat, Artur J.; Gard, Lilli; Fliss, Monika A.; Akkerboom, Viktoria; Benus, Robin F. J.; Lokate, Mariette; Voss, Andreas; Bathoorn, Erik

doi:10.1186/s13756-025-01538-1

Antimicrobial Resistance & Infection Control

Table 1 Summary of E. faecium isolates evaluated by WGS and outbreak specific PCRs, including the isolate name, source, date of isolation, and their antibiotic susceptibility or resistance. Antibiotics to which all isolates were either resistant or susceptible have been excluded from the table

From: Development of a strain-specific PCR as a diagnostic tool for surveillance, detection, and monitoring of vancomycin-resistant Enterococcus faecium during outbreak

Isolate name	Source^a	Date of isolation	Imipenem	Vancomycin	Teicoplanin	Nitrofurantoin
NN-EF1	UMCG	27-8-2021	>= 16.0	<= 0.5	<= 0.5	64
NN-EF2	UMCG	25-9-2021	>= 16.0	<= 0.5	<= 0.5	64
NN-EF3	UMCG	1-10-2021	>= 16.0	<= 0.5	<= 0.5	32
NN-EF4	UMCG	5-10-2021	>= 16.0	<= 0.5	<= 0.5	64
NN-EF5	UMCG	27-10-2021	>= 16.0	<= 0.5	<= 0.5	32
NN-EF6	UMCG	13-11-2021	>= 16.0	<= 0.5	<= 0.5	64
NN-EF7	UMCG	31-1-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF8	UMCG	22-3-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF9	UMCG	6-5-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF10	UMCG	1-6-2022	>= 16.0	8	<= 0.5	32
NN-EF11	UMCG	13-6-2022	>= 16.0	8	<= 0.5	64
NN-EF12	UMCG	12-8-2022	>= 16.0	8	<= 0.5	32
NN-EF13	UMCG	18-8-2022	>= 16.0	<= 0.5	<= 0.5	32
NN-EF14	UMCG	27-9-2022	>= 16.0	<= 0.5	<= 0.5	32
NN-EF15	UMCG	14-11-2022	>= 16.0	<= 0.5	<= 0.5	<= 16.0
NN-EF16	UMCG	21-11-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF17	UMCG	26-11-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF18	UMCG	28-11-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF19	UMCG	5-12-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF20	UMCG	6-12-2022	>= 16.0	<= 0.5	1	64
NN-EF21	UMCG	11-12-2022	>= 16.0	<= 0.5	<= 0.5	64
NN-EF22	UMCG	12-12-2022	>= 16.0	8	<= 0.5	32
NN-EF23	CERTE	9-1-2023	>= 16.0	<= 0.5	<= 0.5	<= 16.0
NN-EF24	UMCG	10-1-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF25	UMCG	24-1-2023	<= 1.0	>= 32.0	>= 32.0	<= 16.0
NN-EF26	CERTE	4-2-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF27	UMCG	9-2-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF28	UMCG	14-2-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF29	CERTE	17-2-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF30	UMCG	24-2-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF31	UMCG	28-2-2023	>= 16.0	<= 0.5	<= 0.5	<= 16.0
NN-EF32	UMCG	14-3-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF33	UMCG	21-3-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF34	UMCG	24-3-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF35	CERTE	31-3-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF36	CERTE	9-4-2023	>= 16.0	8	<= 0.5	32
NN-EF37	UMCG	17-4-2023	>= 16.0	8	<= 0.5	32
NN-EF38	CERTE	14-5-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF39	CERTE	15-5-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF40	UMCG	16-5-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF41	CERTE	4-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF42	CERTE	5-6-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF43	CERTE	6-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF44	UMCG	6-6-2023	>= 16.0	8	<= 0.5	64
NN-EF45	CERTE	8-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF46	CERTE	7-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF47	CERTE	12-6-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF48	UMCG	13-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF49	CERTE	14-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF50	CERTE	29-6-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF51	CERTE	23-5-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF52	CERTE	26-5-2023	>= 16.0	<= 0.5	<= 0.5	32
NN-EF53	UMCG	24-10-2023	>= 16.0	<= 0.5	<= 0.5	64
NN-EF54	UMCG	22-11-2023	>= 16.0	8	<= 0.5	64
NN-EF55	UMCG	4-1-2024	>= 16.0	8	<= 0.5	32

^a Isolates from secondary hospitals were submitted to CERTE for analysis, a medical center in the Northern Netherlands that provides specialized medical diagnostics and advice

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